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fadH [2019-05-24 09:32:17]
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fadH [2019-05-24 09:32:17]

2,4-dienoyl-CoA reductase
locus
BSU14060
pI
6.49
mw
26.98 kDa
protein length
254 aa Sequence Blast
gene length
765 bp Sequence Blast
function
fatty acid degradation
product
2,4-dienoyl-CoA reductase
essential
no
ec
1.3.1.34
synonyms
ykuF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

    • Gene

    Coordinates
    1,478,072 → 1,478,836

    The protein

    Catalyzed reaction/ biological activity

  • Trans-2,3-didehydroacyl-CoA + NADP+ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH (according to Swiss-Prot)

    • Protein family

  • [SW|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|EAEEA4DD9641919830A81185333A2610B964D37C|YhdF]
  • [protein|D9D1FCBFC62F93CAA34DF61A784406F4E9EE6768|YjdA]:
  • [protein|4DEFC2998464BF8327578C36A13A10DD277F991E|YkvO]:
  • [protein|6047F2493FCE3D2A9BDB1AD88E5926ECEED81036|YhxC]:
  • [protein|B6FF689E65906186F3576B378650D713DB84EDDA|YcdF]:

    • Structure

  • [PDB|3IMF] (from B. anthracis, 64% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|CE990D33A12C50D22506A52E0099F7D97A762D4E|fadH]-[gene|0264C72F95D53F0211B52753DA37B4A8C0E982D2|fadG]-[gene|B46D407B116E358DFB6284466DE5926EB3719C53|ykzU]
    description
    [Pubmed|17189250]

    regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]

    • regulation

  • induced in the presence of long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]
  • expression of the operon is strongly induced during [SW|biofilm formation] [pubmed|31113899]
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    Biological materials

    Mutant

  • MGNA-B336 (ykuF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1335 NBRP B. subtilis, Japan]
  • BKE14060 (Δ[gene|CE990D33A12C50D22506A52E0099F7D97A762D4E|fadH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCCAATTG, downstream forward: _UP4_TAATCTGAATATTTATAAGT
  • BKK14060 (Δ[gene|CE990D33A12C50D22506A52E0099F7D97A762D4E|fadH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCCAATTG, downstream forward: _UP4_TAATCTGAATATTTATAAGT

    • References

  • 17189250,17919287